Cell state transition dynamics at single-cell level
Project Lead: Sui Huang
Co-lead: Lee Hood
Project 3 extends the need for digitization of biology from molecules to single cells. In this project, stem cell differentiation to cardiomyocytes will be studied at the single-cell level to identify the discretely distinct cell subpopulations representing the metastable intermediate states in the developmental trajectory to the fully differentiated cardiomyocyte. Sorted (homogeneous) subpopulations afford a novel object for analysis methods that cannot access single cells (e.g., proteomics) and hence offer a new degree of resolution of molecular variables in time courses. The big question is whether successive discrete quantized cell states mediate cardiomyocyte development —or whether the processes employ amorphous stochastic intermediates.