Project Leads: Chris Lausted, Sui Huang

The Single Cell facility houses the Fluidigm system, mainly consisting of the C1 and the Biomark, and the nanostring nCounter system. The Fluidigm C1 functions to isolate single cells and create pre-amplified cDNA for further downstream analysis. The Fluidigm Biomark produces high-throughput gene expression data. The nanostring nCounter system is capable of generating sensitive single cell transcript counts with minimal amplification based on, up to, 800 genes.

. Identification of regulators that orchestrate post-differentiation transitions. A) Basic concept of our study design: combine population dynamics with gene regulatory modules to understand how cell-to-cell variability and “quantized” populations are regulated through the Gene Regulatory Network. B) Heatmap of the gene expression levels of 34 differentially regulated transcripts in post-differentiation day 7 and 21

Identification of regulators that orchestrate post-differentiation transitions.